Stereotaxic assembly and procedures for simultaneous electrophysiological and MRI study of conscious rat

Khubchandani, M. ; Mallick, H. N. ; Jagannathan, N. R. ; Mohan Kumar, V. (2003) Stereotaxic assembly and procedures for simultaneous electrophysiological and MRI study of conscious rat Magnetic Resonance in Medicine, 49 (5). pp. 962-967. ISSN 0740-3194

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/mrm.104...

Related URL: http://dx.doi.org/10.1002/mrm.10441

Abstract

A stereotaxic restraining assembly was designed and developed for simultaneous electrophysiological recordings and functional MRI (fMRI) data acquisition from a conscious rat. The design of the nonmagnetic stereotaxic apparatus facilitated the restraining of head and body of the unanesthetized conscious animal during MRI experiments. The apparatus was made of Teflon and Perspex materials with an appropriate size and shape for a 4.7 T / 40 cm animal MRI scanner. Electrodes made from nonmagnetic silver wire were implanted on the skull for recording the electroencephalogram (EEG), the electro-oculogram (EOG), and the electromyogram (EMG), while polycarbonate screws were used for anchoring the electrode assembly. There were no major distortions or artifacts observed in the electrophysiological tracings and MR images. Electrophysiological recordings during fMRI acquisitions are useful to study different neurophysiological mechanisms of sleep and pathophysiology of seizure activity. Integration of electrophysiological recordings (with their good temporal resolution) and MRI (with its superior spatial resolution) is helpful in characterizing the functional state of different brain regions.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Stereotaxi; Design; Fabrication; Electrophysiology; Sleep; Conscious Animal; fMRI
ID Code:65865
Deposited On:19 Oct 2011 14:29
Last Modified:19 Oct 2011 14:29

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