Quantification of binary diffusion in protein crystals

Cvetkovic, Aleksandar ; Picioreanu, Cristian ; Straathof, Adrie J. J. ; Krishna, Rajamani ; van der Wielen, Luuk A. M. (2005) Quantification of binary diffusion in protein crystals The Journal of Physical Chemistry B, 109 (21). pp. 10561-10566. ISSN 1089-5647

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Official URL: http://pubs.acs.org/doi/abs/10.1021/jp050289c

Related URL: http://dx.doi.org/10.1021/jp050289c


The use of confocal laser scanning microscopy for visualization and quantification of binary diffusion within anisotropic porous material is described here for the first time. The dynamics of adsorption profiles of dianionic fluorescein, zwitterionic rhodamine B, and their mixture in the cationic native orthorhombic lysozyme crystal were subsequently analyzed. All data could be described by a classical pore diffusion model. There was no change in the adsorption characteristics, but diffusion decreased with the introduction of a second solute in the solution. It was found that diffusion is determined by the combination of steric and electrostatic interactions, while adsorption is dependent on electrostatic and hydrophobic interactions. Thus, it was established that the outcome of binary transport depends on the solute, protein, and crystal characteristics.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:65384
Deposited On:17 Oct 2011 03:22
Last Modified:17 Oct 2011 03:22

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