Picloram induced somatic embryogenesis in chickpea (Cicer arietinum L.)

Dineshkumar, V. ; Kirti, P. B. ; Sachan, J. K. S. ; Chopra, V. L. (1995) Picloram induced somatic embryogenesis in chickpea (Cicer arietinum L.) Plant Science, 109 (2). pp. 207-213. ISSN 0168-9452

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/016894...

Related URL: http://dx.doi.org/10.1016/0168-9452(95)04167-S


Complete plantlets were regenerated via somatic embryogenesis in the widely cultivated chickpea variety BG256. Leaf explants, from 13 day-old axenic seedlings yielded somatic embryos with high efficiency during dark incubation on MS medium supplemented with B5 vitamins, 0.25 mg/l each of 2,4-D and picloram, and 0.1 mg/l BAP. A modified B5 medium with 2 mg/l BAP and 0.1 mg/l IAA was found suitable for maturation of the embryos. The well-formed embryos germinated on basal B5 medium supplemented with 0.25 mg/l BAP and developed into healthy plantlets on basal B5 medium. Hardened plantlets grew into normal, fertile pod bearing plants upon transfer to soil.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Somatic Embryogenesis; Grain Legume; Chickpea (Cicer arietinum L); Picloram; Normal Regenerants
ID Code:6527
Deposited On:20 Oct 2010 11:03
Last Modified:28 May 2011 06:41

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