A Moricandia arvensis- based cytoplasmic male sterility and fertility restoration system in Brassica juncea

Prakash, S. ; Kirti, P. B. ; Bhat, S. R. ; Gaikwad, K. ; Kumar, V. D. ; Chopra, V. L. (1998) A Moricandia arvensis- based cytoplasmic male sterility and fertility restoration system in Brassica juncea Theoretical and Applied Genetics, 97 (3). pp. 488-492. ISSN 0040-5752

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Official URL: http://www.springerlink.com/content/awqyj50nxpwych...

Related URL: http://dx.doi.org/10.1007/s001220050921

Abstract

A cytoplasmic male-sterility system has been developed in mustard (Brassica juncea) following repeated backcrossings of the somatic hybrid Moricandia arvensis (2n=28, MM)+B. juncea (2n=36, AABB), carrying mitochondria and chloroplasts from M. arvensis, to Brassica juncea. Cytoplasmic male-sterile (CMS) plants are similar to normal B. juncea; however, the leaves exhibit severe chlorosis resulting in delayed flowering. Flowers are normal with slender, non-dehiscent anthers and excellent nectaries. CMS plants show regular meiosis with pollen degeneration occurring during microsporogenesis. Female fertility was normal. Genetic information for fertility restoration was introgressed following the development of a M. arvensis monosomic addition line on CMS B. juncea. The additional chromosome paired allosyndetically with one of the B. juncea bivalents and allowed introgression. The putative restorer plant also exhibited severe chlorosis similar to CMS plants but possessed 89% and 73% pollen and seed fertility, respectively, which subsequently increased to 96% and 87% in the selfed progeny. The progeny of the cross of CMS line with the restorer line MJR-15, segregated into 1 fertile : 1 sterile. The CMS (Moricandia) B. juncea, the restorer (MJR-15), and fertility restored F1 plants possess similar cytoplasmic organellar genomes as revealed by 'Southern' analysis.

Item Type:Article
Source:Copyright of this article belongs to Springer-Verlag.
Keywords:Cytoplasmic Male Sterility; Fertility Restoration; Moricandia arvensis; Brassica juncea; Protoplast Fusion; Somatic Hybrids
ID Code:6525
Deposited On:20 Oct 2010 11:02
Last Modified:28 May 2011 06:21

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