Fluorescence quenching of biologically active carboxamide by aniline and carbon tetrachloride in different solvents using Stern-Volmer plots

Abstract

Fluorescence quenching of biologically active carboxamide namely (E)-2-(4-chlorobenzylideneamino)- N-(2-chlorophenyl)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxamide [ECNCTTC] by aniline and carbon tetrachloride (CCl₄) quenchers in different solvents using steady state method and time resolved method using only one solvent has been carried out at room temperature to understand the role of quenching mechanisms. The Stern-Volmer plot has been found to be linear for all the solvents studied. The probability of quenching per encounter p (p') was determined in all the solvents and was found to be less than unity. Further, from the studies of rate parameters and life time measurements in n-heptane and cyclohexane with aniline and carbon tetrachloride as quenchers have been shown that, the phenomenon of quenching is generally governed by the well-known Stern-Volmer (S-V) plot. The activation energy E_a (or E'_a) of quenching was determined using the literature values of activation energy of diffusion Ed and the experimentally determined values of p (or p'). It has been found that, the activation energy E_a (E'_a) is greater than the activation energy for diffusion Ed in all solvents. Hence, from the magnitudes of E_a (or E'_a) as well as p (or p') infer that, the quenching mechanism is not solely due to the material diffusion, but there is also contribution from the activation energy.