Transcriptomic and proteomic profile of Aspergillus fumigatus on exposure to artemisinin

Gautam, Poonam ; Upadhyay, Santosh Kumar ; Hassan, Wazid ; Madan, Taruna ; Sirdeshmukh, Ravi ; Sundaram, Curam Sreenivasacharlu ; Gade, Wasudev Namdeo ; Basir, Seemi Farhat ; Singh, Yogendra ; Sarma, Puranam Usha (2011) Transcriptomic and proteomic profile of Aspergillus fumigatus on exposure to artemisinin Mycopathologia, 172 (5). pp. 331-346. ISSN 0301-486X

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Official URL: http://www.springerlink.com/content/13718277583173...

Related URL: http://dx.doi.org/10.1007/s11046-011-9445-3

Abstract

Artemisinin, an antimalarial drug, and its derivatives are reported to have antifungal activity against some fungi. We report its antifungal activity against Aspergillus fumigatus (A. fumigatus), a pathogenic filamentous fungus responsible for allergic and invasive aspergillosis in humans, and its synergistic effect in combination with itraconazole (ITC), an available antifungal drug. In order to identify its molecular targets, we further analyzed transcript and proteomic profiles of the fungus on exposure to the artemisinin. In transcriptomic analysis, a total of 745 genes were observed to be modulated on exposure to artemisinin, and some of them were confirmed by real-time polymerase chain reaction analysis. Proteomic profiles of A. fumigatus treated with artemisinin showed modulation of 175 proteins (66 upregulated and 109 downregulated) as compared to the control. Peptide mass fingerprinting led to the identification of 85 proteins — 29 upregulated and 56 downregulated, 65 of which were unique proteins. Consistent with earlier reports of molecular mechanisms of artemisinin and that of other antifungal drugs, we believe that oxidative phosphorylation pathway (64 kDa mitochondrial NADH dehydrogenase), cell wall-associated proteins and enzymes (conidial hydrophobin B protein, cell wall phiA protein, extracellular thaumatin domain protein, 1,3-beta-glucanosyltransferase Gel2) and genes involved in ergosterol biosynthesis (ERG6 and coproporphyrinogen III oxidase, HEM13) are potential targets of artemisinin for further investigations.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Aspergillus fumigatus; Artemisinin; Microarray; Proteomics
ID Code:64870
Deposited On:15 Oct 2011 12:51
Last Modified:18 Jun 2012 11:32

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