A stable cytoplasmic male-sterile line of Brassica juncea carrying restructured organelle genomes from the somatic hybrid Trachystoma ballii+B. juncea

Kirti, P. B. ; Mohapatra, T. ; Baldev, A. ; Prakash, S. ; Chopra, V. L. (1995) A stable cytoplasmic male-sterile line of Brassica juncea carrying restructured organelle genomes from the somatic hybrid Trachystoma ballii+B. juncea Plant Breeding, 114 (5). pp. 434-438. ISSN 0179-9541

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-...

Related URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00826.x

Abstract

A cytoplasmic male-sterile (CMS) line of Brassica juncea has been developed by combining the cytoplasm originating from the somatic hybrid Trachystoma ballii+B. juncea, and the nucleus of B. juncea cv. Pusa Bold by repeated backcrossing. Male-sterile plants closely resembled the normal fertile B. juncea in general morphology, but had delayed flowering (5-7 days) when compared with fertile 'Pusa Bold' which flowered in 45 days. Stamens of the male-sterile line were transformed into petaloid structures. Pollen abortion occurred after tetrad formation. Female fertility of the male-sterile line was normal. Molecular analysis of organelle genomes indicated extensive mitochondrial DNA recombinations in the CMS line. Preliminary analysis of the chloroplast genome of the CMS line also indicated chloroplast DNA recombination.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Brassica Juncea; Trachystoma Ballii; Cytoplasmic Male-sterility; Chloroplast Dna Recombination; Mitochondrial Dna Recombination; Somatic Hybrid
ID Code:6474
Deposited On:20 Oct 2010 10:22
Last Modified:28 May 2011 06:40

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