Kirti, P. B. ; Chopra, V. L. (1989) A simple method of inducing somatic embryogenesis in Brassica juncea (L.) Czern & Coss. Plant Breeding, 102 (1). pp. 73-78. ISSN 0179-9541
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-...
Related URL: http://dx.doi.org/10.1111/j.1439-0523.1989.tb00317.x
Abstract
A single-step method for the induction and development of somatic embryoids from hypocotyls explains of Brassica juncea is reported. On modified MS medium containing 2 % sucrose, 0.25 mgl 1 2,4-D, 0.5 mgl 1 each of NAA and BaP-R, each explant calluses at both of and at its best, 31% of explants produce embryoids. In the variety RLM-198, the number of embryoids ranges from 8-21 per culture. Each embryoid, upon proliferation, developed up to the 25 shoots. The method is rapid; the time La ken from inoculation to the development of intact plantlets is 8-10 weeks. Regenerated plants have flowered normally and have set seed. The system can profitably be used for in vitro mutant selection and early bulking in mustard.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
| Keywords: | Brassica juncea; Hypocotyl Explants; Somatic Embryogenesis; Plant Regeneration |
| ID Code: | 6472 |
| Deposited On: | 20 Oct 2010 10:21 |
| Last Modified: | 28 May 2011 07:00 |
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