Nagesh, Narayana ; Chatterji, Dipankar (1997) A useful method for the synthesis of 8 azido-guanosine tri- and tetraphosphate: important substrates for many enzymatic reactions Journal of Biochemical and Biophysical Methods, 35 (01). pp. 61-66. ISSN 0165-022X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01650...
Related URL: http://dx.doi.org/10.1016/S0165-022X(97)00013-4
Abstract
8-N3GMP was synthesised from 8-BrGMP by the addition of LiN3. 8-N3GMP was then phosphorylated to N3-GDP and N3-GTP by controlled enzymatic reaction. 8-N3GDP can be converted to N3-ppGpp with crude Rel A, which phosphorylates the 3'-OH of GDP.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | N3-GTP; N3-ppGpp; Synthesis; Purification; Phenyl Boronate Column |
| ID Code: | 6465 |
| Deposited On: | 20 Oct 2010 10:11 |
| Last Modified: | 08 Feb 2011 05:58 |
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