An RNA helicase, CrhR, regulates the low-temperature-inducible expression of heat-shock genes groES, groEL1 and groEL2 in Synechocystis sp. PCC 6803

Prakash, Jogadhenu S. S. ; Krishna, Pilla Sankara ; Sirisha, Kodru ; Kanesaki, Yu ; Suzuki, Iwane ; Shivaji, Sisinthy ; Murata, Norio (2010) An RNA helicase, CrhR, regulates the low-temperature-inducible expression of heat-shock genes groES, groEL1 and groEL2 in Synechocystis sp. PCC 6803 Microbiology, 156 (2). pp. 442-451. ISSN 1350-0872

Full text not available from this repository.

Official URL: http://mic.sgmjournals.org/content/156/2/442.short

Related URL: http://dx.doi.org/10.1099/mic.0.031823-0

Abstract

The crhR gene for RNA helicase, CrhR, was one of the most highly induced genes when the cyanobacterium Synechocystis sp. PCC 6803 was exposed to a downward shift in ambient temperature. Although CrhR may be involved in the acclimatization of cyanobacterial cells to low-temperature environments, its functional role during the acclimatization is not known. In the present study, we mutated the crhR gene by replacement with a spectinomycin-resistance gene cassette. The resultant ΔcrhR mutant exhibited a phenotype of slow growth at low temperatures. DNA microarray analysis of the genome-wide expression of genes, and Northern and Western blotting analyses indicated that mutation of the crhR gene repressed the low-temperature-inducible expression of heat-shock genes groEL1 and groEL2, at the transcript and protein levels. The kinetics of the groESL co-transcript and the groEL2 transcript after addition of rifampicin suggested that CrhR stabilized these transcripts at an early phase, namely 5-60 min, during acclimatization to low temperatures, and enhanced the transcription of these genes at a later time, namely 3-5 h. Our results suggest that CrhR regulates the low-temperature-inducible expression of these heat-shock proteins, which, in turn, may be essential for acclimatization of Synechocystis cells to low temperatures.

Item Type:Article
Source:Copyright of this article belongs to Society for General Microbiology.
ID Code:64376
Deposited On:08 Oct 2011 04:11
Last Modified:08 Oct 2011 04:11

Repository Staff Only: item control page