Alam, S. I. ; Dube, Smita ; Reddy, G. S. N. ; Bhattacharya, B. K. ; Shivaji, S. ; Singh, Lokendra (2005) Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica Enzyme and Microbial Technology, 36 (5-6). pp. 824-831. ISSN 0141-0229
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.enzmictec.2005.01.011
Abstract
One anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate was psychrotrophic forming maximum cell mass between 5 and 10 °C and produced extracellular protease. Growth was observed in the pH range of 6.5-8.5 with optimum at pH 8. Protease was purified 12.7-fold with a total yield of 26.2%. Effect of temperature, pH and salt concentration on enzyme activity were studied. Protease was found to be a serine-type metaloenzyme, which is active in a broad range of pH. It was moderately thermolabile and resistant to SDS. Enzyme kinetics revealed a tendency to decrease Km with increase in temperature for casein substrate.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Clostridium; Psychrotroph; Antarctica; Protease; Anaerobe |
| ID Code: | 64329 |
| Deposited On: | 07 Oct 2011 12:40 |
| Last Modified: | 07 Oct 2011 12:40 |
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