Reddy, G. S. N. ; Rajagopalan, G. ; Shivaji, S. (1994) Thermolabile ribonucleases from antarctic psychrotrophic bacteria: detection of the enzyme in various bacteria and purification from Pseudomonas fluorescens FEMS Microbiology Letters, 122 (3). pp. 211-216. ISSN 0378-1097
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...
Related URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07169.x
Abstract
Thirteen terrestrial psychrotrophic bacteria from Antarctica were screened for the presence of a thermolabile ribonuclease (RNAase-HL). The enzyme was detected in three isolates of Pseudomonas fluorescens and one isolate of Pseudomonas syringae. It was purified from one P. fluorescens isolate and the molecular mass of the enzyme as determined by SDS-PAGE was 16 kDa. RNAase-HL exhibited optimum activity around 40°C at pH 7.4. It could hydrolyse Escherichia coli RNA and the synthetic substrates poly(A), poly(C), poly(U) and poly(A-U). Unlike the crude RNAase from mesophilic P. fluorescens and pure bovine pancreatic RNAase A which were active even at 65°C, RNAase-HL was totally and irreversibly inactivated at 65°C.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to John Wiley and Sons. |
| Keywords: | Thermolabile Ribonuclease; RNAase; Antarctic Psychotrophic Bacteria; Pseudomonas fluorescens; Pseudomonas syringae |
| ID Code: | 64325 |
| Deposited On: | 07 Oct 2011 12:37 |
| Last Modified: | 07 Oct 2011 12:37 |
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