Chintalapati, Suresh ; Prakash, J. S. S. ; Singh, Ashish K. ; Ohtani, Shuji ; Suzuki, Iwane ; Murata, Norio ; Shivaji, Sisinthy (2007) Desaturase genes in a psychrotolerant Nostoc sp. are constitutively expressed at low temperature Biochemical and Biophysical Research Communications, 362 (1). pp. 81-87. ISSN 0006-291X
Full text not available from this repository.
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.bbrc.2007.07.150
Abstract
Antarctic psychrotolerant, Nostoc sp. (SO-36), when grown at 25 °C and then shifted to 10 °C, showed an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids. These results indicate that the activities of the enzymes DesA and DesB are up-regulated, when cultures were grown at 10 °C or shifted to 10 °C from 25 °C. However, RT-PCR studies indicated a constitutive expression of desA, desB, desC, and desC2 genes when cultures grown at 25 °C were shifted to 10 °C. This constitutive expression of des genes is in contrast to that observed in mesophilic cyanobacteria, in which desA and desB are transcriptionally up-regulated in response to lowering of growth temperature.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Cyanobacteria; Nostoc sp.; Desaturases; Des Genes; Constitutive Expression; Antarctica; Psychrotolerant; Fatty Acid Composition; RT-PCR |
| ID Code: | 64309 |
| Deposited On: | 08 Oct 2011 04:08 |
| Last Modified: | 08 Oct 2011 04:08 |
Repository Staff Only: item control page
