Expression of male sterility in alloplasmic Brassica juncea with Erucastrum canariense cytoplasm and the development of a fertility restoration system

Prakash, S. ; Ahuja, I. ; Upreti, H. C. ; Dinesh Kumar, V. ; Bhat, S. R. ; Kirti, P. B. ; Chopra, V. L. (2001) Expression of male sterility in alloplasmic Brassica juncea with Erucastrum canariense cytoplasm and the development of a fertility restoration system Plant Breeding, 120 (6). pp. 479-482. ISSN 0179-9541

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Official URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1439-...

Related URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00627_x

Abstract

An alloplasmic mustard, Brassica juncea, has been synthesized by placing its nucleus into the cytoplasm of the related wild species Erucastrum canariense to express cytoplasmic male sterility. To achieve this, the sexual hybrid E. canariense (2n=18, EcEc) ×Brassica campestris (2n= 20, AA) was repeatedly backcrossed to B. juncea (2n= 36, AABB). Cytoplasmic male-sterile (CMS) plants were recovered in the BC4 generation. These plants are a normal green and the flowers have slender, non-dehiscing anthers that contain sterile pollen. Nectaries are well developed and female fertility is > 90%. The fertility restoration gene was introgressed to CMS B. juncea from the cytoplasmic donor E. canariense through pairing between chromosomes belonging to B. juncea with those of the E. canariense genome. The restorer plants have normal flowers, with well-developed anthers containing fertile pollen. Meiosis proceeds normally. Pollen and seed fertility averaged 90% and 82%, respectively. F1 hybrids between CMS and the restorer are fully pollen fertile and show normal seed set. Preliminary results indicate that restoration is achieved by a single dominant gene. The constitution of the organelle genomes of the CMS, restorer and fertility restored plants is identical, as revealed by Southern analysis using mitochondrial and chloroplast probes atp A and psb D, respectively.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons, Inc.
Keywords:Brassica Juncea; Erucastrum Canariense; Alloplasmic Hybrid; Cytoplasmic Male Sterility; Fertility Restorer; Interspecific Cross
ID Code:6427
Deposited On:20 Oct 2010 10:25
Last Modified:28 May 2011 06:20

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