A reverse passive haemagglutination test for detection of Japanese encephalitis virus antigens in cerebrospinal fluid

Ravi, V. ; Premkumar, S. ; Chandramuki, A. ; Kimura-Kuroda, J. (1989) A reverse passive haemagglutination test for detection of Japanese encephalitis virus antigens in cerebrospinal fluid Journal of Virological Methods, 23 (3). pp. 291-298. ISSN 0166-0934

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0166-0934(89)90161-4

Abstract

A rapid sensitive and specific reverse passive haemagglutination test (RPHA) was developed for the detection of Japanese encephalitis virus (JEV) antigens in human cerebrospinal fluid (CSF). Sheep red cells were sensitized with five monoclonal antibodies (109, 112, 203, 204 and 301) reactive with envelope glycoprotein of JEV. Viral antigens were detected in CSF from 35 of 58 (60%) clinical cases of JE when the five MAb coated cells were used in combination. An IgM capture ELISA detected JEV specific antibodies in CSF among 52 of these 58 cases (90%). While 29 specimens contained both antigen and IgM antibodies, 23 had only IgM antibodies and 6 had only antigen. RPHA proved valuable for detection of viral antigens in CSF samples obtained within 10 days after onset of clinical symptoms. Amongst the five MAbs used, the individual antigen detection rates were 44, 12, 43, 12 and 36%, for MAbs 109, 112. 203. 204 and 301. respectively.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Japanese Encephalitis; Viral Antigen; Cerebrospinal Fluid; Monoclonal Antibody; Reverse Passive Hemagglutination
ID Code:63657
Deposited On:29 Sep 2011 06:26
Last Modified:29 Sep 2011 06:26

Repository Staff Only: item control page