Das, S. K. ; Sedelnikova, S. E. ; Baker, P. J. ; Ruzheinikov, S. N. ; Foster, S. ; Hartley, A. ; Horsburgh, M. J. ; Rice, D. W. (2001) Cloning, purification, crystallization and preliminary crystallographic analysis of Bacillus subtilis LuxS Acta Crystallographica Section D, D57 (9). pp. 1324-1325. ISSN 0907-4449
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Official URL: http://scripts.iucr.org/cgi-bin/paper?S09074449010...
Related URL: http://dx.doi.org/10.1107/S0907444901011611
Abstract
LuxS of Bacillus subtilis is a member of a novel family of proteins with a potential role in quorum sensing, controlling important aspects of cellular physiology in a range of microbial species. B. subtilis luxS was cloned, expressed in Escherichia coli, purified and crystallized using the hanging-drop method of vapour diffusion with ammonium sulfate as the precipitant. The crystals belong to one of the enantiomorphic space groups P6122 or P6522, with approximate unit-cell parameters α = b = 63.6, c = 151.5 Å and one subunit in the asymmetric unit, corresponding to a packing density of 2.5 Å3 Da-1. The crystals diffract X-rays to at least 1.55 Å resolution on a synchrotron-radiation source. Determination of the structure will provide insights into the key determinants of function of this class of proteins, for which no structures are currently available.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to International Union of Crystallography. |
| Keywords: | LuxS; Quorum Sensing; Bacillus subtilis |
| ID Code: | 63477 |
| Deposited On: | 29 Sep 2011 04:22 |
| Last Modified: | 29 Sep 2011 04:22 |
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