A collagenase assay using [3H-methyl]collagen

Bhatnagar, Rakesh ; Decker, Karl (1981) A collagenase assay using [3H-methyl]collagen Journal of Biochemical and Biophysical Methods, 5 (3). pp. 147-152. ISSN 0165-022X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0165-022X(81)90013-0


A new assay procedure for collagenase is presented. Highly radioactive substrate is prepared by methylation of native collagen. The 3H-labelled protein is readily attacked by bacterial as well as by mammalian collagenase and resistant to other proteinases. The sensitivity of this assay is higher than that of the enzymic methods hitherto available.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Collagenase Assay; Collagen
ID Code:63379
Deposited On:28 Sep 2011 10:33
Last Modified:28 Sep 2011 10:33

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