Arabidopsis mutants deficient in T-DNA integration

Sonti, R. V. ; Chiurazzi, M. ; Wong, D. ; Davies, C. S. ; Harlow, G. R. ; Mount, D. W. ; Signer, E. R. (1995) Arabidopsis mutants deficient in T-DNA integration PNAS, 92 (25). pp. 11786-11790. ISSN 0027-8424

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Arabidopsis thaliana mutants originally isolated as hypersensitive to irradiation were screened for the ability to be transformed by Agrobacterium transferred DNA (T-DNA). One of four UV-hypersensitive mutants and one of two gamma-hypersensitive mutants tested showed a significant reduction in the frequency of stable transformants compared with radioresistant controls. In a transient assay for T-DNA transfer independent of genomic integration, both mutant lines took up and expressed T-DNA as efficiently as parental lines. These lines are therefore deficient specifically in stable T-DNA integration and thus provide direct evidence for the role of a plant function in that process. As radiation hypersensitivity suggests a deficiency in repair of DNA damage, that plant function may be one that is also involved in DNA repair, possibly, from other evidence, in repair of double-strand DNA breaks.

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ID Code:62634
Deposited On:22 Sep 2011 02:47
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