Dynamics of membrane nanotubulation and DNA self-assembly

Roopa, T. ; Kumar, N. ; Bhattacharya, S. ; Shivashankar, G. V. (2004) Dynamics of membrane nanotubulation and DNA self-assembly Biophysical Journal, 87 (2). pp. 974-979. ISSN 0006-3495

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1529/biophysj.103.039297

Abstract

A localized point-like force applied perpendicular to a vesicular membrane layer, using an optical tweezer, leads to membrane nanotubulation beyond a threshold force. Below the threshold, the force-extension curve shows an elastic response with a fine structure (serrations). Above the threshold the tubulation process exhibits a new reversible flow phase for the multilamellar membrane, which responds viscoelastically. Furthermore, with an oscillatory force applied during tubulation, broad but well-resolved resonances occur in the flow phase, presumably matching the time scales associated with the vesicle-nanotubule coupled system. These nanotubules, anchored to the optical tweezer also provide, for the first time, a direct probe of the real-time dynamics of DNA self-assembly on membranes. Our studies are a step in the direction of analyzing the dynamics of membrane self-assembly and artificial nanofluidic membrane networks.

Item Type:Article
Source:Copyright of this article belongs to Biophysical Society.
ID Code:62266
Deposited On:20 Sep 2011 09:36
Last Modified:18 May 2016 11:38

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