Comparison of nuclear and polysomal RNA fractions from goat brain

Chaudhury, S. ; Bhattacharya, M. ; Sarkar, P. K. (1980) Comparison of nuclear and polysomal RNA fractions from goat brain Biochemical and Biophysical Research Communications, 92 (4). pp. 1130-1135. ISSN 0006-291X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0006-291X(80)90404-0

Abstract

Phenol extracted RNA preparations from highly purified nuclei and polysomes of goat brain were fractionated by chromatography on oligo (dT)-cellulose and analyzed by electrophoresis on agarose-acrylamide composite gels. The electrophoretic profile of the polysomal polyadenylated RNA fraction showed a major band with a molecular weight of about 0.62×106, which corresponds to the size of the tubulin mRNA. The nuclear polyadenylated RNA fraction also displayed a single major band, with an estimated molecular weight of 0.76×106, which appears to be a potential precursor of tubulin mRNA.

Item Type:Article
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ID Code:61678
Deposited On:15 Sep 2011 12:20
Last Modified:15 Sep 2011 12:20

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