Pituitary gonadotropins regulate spermatogonial differentiation and proliferation in the rat

Shetty, Jagatpala ; Matathe, Gopal E. ; Ramaswamy, Suresh ; Dighe, Rajan R. (1996) Pituitary gonadotropins regulate spermatogonial differentiation and proliferation in the rat Journal of Biosciences, 21 (1). pp. 81-92. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/21/81-92.pdf

Related URL: http://dx.doi.org/10.1007/BF02716814


The relative regulatory roles of the pituitary gonadotropins, luteinizing hormone and follicle stimulating hormone in the spermatogonial proliferation has been studied using specific antibodies against these hormones in the immature rats. Immunoneutralization of luteinizing hormone for 7 days resulted in significant reduction in tetraploid cells and total absence of haploid cells, while there was a relative increase in the diploid population. This was also accomopanied by a decrease in spermatogonial proliferation as indicated by a decrease in [3H] thymidine incorporation into DNA by purified spermatogonia. Administration of follicle stimulating hormone a/s for 7 days also caused a significant decrease in the rate of spermatogonial proliferation. Withdrawal of follicle stimulating hormone led to a significant reduction in tetraploid and haploid cells. However interestingly, it failed to totally abolish the appearance of these cells. Administration of testosterone (3 mg/day/rat) for 2 days along with the gonadotropin a/s could partially reverse the effect on spermatogonial proliferation. It is concluded that (i) both luteinizing hormone and follicle stimulating hormone are involved in spermatogonial proliferation, (ii) lack of testosterone consequent of the neutralization of luteinizing hormone prevented the entry of spermatogonial cells into meiosis, (iii) testosterone may be involved in spermatogonia] proliferation providing a mitotic signal and (v) both follicle stimulating hormone and testosterone act synergistically and lack of any one of the hormones results in impairment of spermatogonial proliferation.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Spermatogonial Proliferation; Gonadotropins; DNA Synthesis; Antibodies
ID Code:60530
Deposited On:09 Sep 2011 04:16
Last Modified:18 May 2016 10:36

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