Virus-induced gene silencing for functional analysis of selected genes

Godge, Mandar R. ; Purkayastha, Arunima ; Dasgupta, Indranil ; Kumar, Prakash P. (2008) Virus-induced gene silencing for functional analysis of selected genes Plant Cell Reports, 27 (2). pp. 209-219. ISSN 0721-7714

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Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:VIGS; Viral Vectors; Gene Silencing; Functional Genomics
ID Code:60493
Deposited On:09 Sep 2011 03:42
Last Modified:07 Mar 2012 10:20

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