RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants

Tyagi, Himani ; Rajasubramaniam, Shanmugam ; Rajam, Manchikatla Venkat ; Dasgupta, Indranil (2008) RNA-interference in rice against Rice tungro bacilliform virus results in its decreased accumulation in inoculated rice plants Transgenic Research, 17 (5). pp. 897-904. ISSN 0962-8819

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Official URL: http://www.springerlink.com/content/58g8761t963q45...

Related URL: http://dx.doi.org/10.1007/s11248-008-9174-7

Abstract

Rice tungro is a viral disease seriously affecting rice production in South and Southeast Asia. Tungro is caused by the simultaneous infection in rice of Rice tungro bacilliform virus (RTBV), a double-stranded DNA virus and Rice tungro spherical virus (RTSV), a single-stranded RNA virus. To apply the concept of RNA-interference (RNAi) for the control of RTBV infection, transgenic rice plants expressing DNA encoding ORF IV of RTBV, both in sense as well as in anti-sense orientation, resulting in the formation of double-stranded (ds) RNA, were raised. RNA blot analysis of two representative lines indicated specific degradation of the transgene transcripts and the accumulation of small molecular weight RNA, a hallmark for RNA-interference. In the two transgenic lines expressing ds-RNA, different resistance responses were observed against RTBV. In one of the above lines (RTBV-O-Ds1), there was an initial rapid buildup of RTBV levels following inoculation, comparable to that of untransformed controls, followed by a sharp reduction, resulting in approximately 50-fold lower viral titers, whereas the untransformed controls maintained high levels of the virus till 40 days post-inoculation (dpi). In RTBV-O-Ds2, RTBV DNA levels gradually rose from an initial low to almost 60% levels of the control by 40 dpi. Line RTBV-O-Ds1 showed symptoms of tungro similar to the untransformed control lines, whereas line RTBV-O-Ds2 showed extremely mild symptoms.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Transgenic Rice; Rice tungro bacilliform virus; RNAi; Small RNA; Green Leafhopper; Virus Resistance
ID Code:60492
Deposited On:09 Sep 2011 03:42
Last Modified:09 Sep 2011 03:42

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