Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis

Banerjee, Sanjiban K. ; Misra, Parimal ; Bhatt, Kamlesh ; Mande, Shekhar C. ; Chakraborti, Pradip K. (1998) Identification of an ABC transporter gene that exhibits mRNA level overexpression in fluoroquinolone-resistant Mycobacterium smegmatis FEBS Letters, 425 (1). pp. 151-156. ISSN 0014-5793

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0014-5793(98)00214-2

Abstract

We describe here the PCR amplification of a DNA fragment (mtp1) from Mycobacterium smegmatis using primers derived from consensus sequences of the ABC family of transporters. The fragment encodes amino acid sequences that exhibited significant homology with different ABC transporters. Amino acid sequence alignment of the full length gene with other transporters identified the ABC protein as the B-subunit of the phosphate specific transporter. Strikingly, a M. smegmatis colony which exhibited a high level of ciprofloxacin resistance showed mRNA level overexpression of mtp1. Thus this is the first report in any prokaryote indicating differential expression of an ABC transporter in a fluoroquinolone resistant colony.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:ABC Transporter; Active Efflux; Phosphate Specific Transport; Drug Resistance; Fluoroquinolone; Mycobacterium
ID Code:60376
Deposited On:08 Sep 2011 14:36
Last Modified:16 Jul 2012 16:05

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