Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis

Bhatt, Kamlesh ; Banerjee, Sanjiban K. ; Chakraborti, Pradip K. (2001) Evidence that phosphate specific transporter is amplified in a fluoroquinolone resistant Mycobacterium smegmatis European Journal of Biochemistry, 267 (13). pp. 4028-4032. ISSN 0014-2956

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Official URL: http://onlinelibrary.wiley.com/doi/10.1046/j.1432-...

Related URL: http://dx.doi.org/10.1046/j.1432-1327.2000.01437.x

Abstract

We reported in an earlier study that active efflux of drug has a predominant role in conferring resistance in a laboratory-generated ciprofloxacin-resistant mutant of Mycobacterium smegmatis. This mutant exhibited mRNA level overexpression, as well as chromosomal amplification, of the gene pstB, encoding the putative ATPase subunit of phosphate specific transport (Pst) system. We demonstrate here that this mutant shows enhanced phosphate uptake and that inactivation of pstB in the parental strain results in loss of high affinity phosphate uptake and hypersensitivity to fluoroquinolones. These findings suggest a novel role of the Pst system in active efflux, in addition to its involvement in phosphate transport.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:ABC Transporter; Drug Resistance; Fluoroquinolone; Mycobacteria; Phosphate Specific Transporter
ID Code:60358
Deposited On:08 Sep 2011 14:37
Last Modified:08 Sep 2011 14:37

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