The cloning and expression of transfer RNA gene cluster of Vibrio eltor phage e4

Chattopadhyay, Samit ; Ghosh, Ranajit Kumar (1989) The cloning and expression of transfer RNA gene cluster of Vibrio eltor phage e4 Virology, 171 (1). pp. 114-119. ISSN 0042-6822

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0042-6822(89)90517-5

Abstract

Vibrio eltor phage e4 codes for five different tRNA species [Chattopadhyay and Ghosh, 1988b]. The tRNA genes contained in a 3.4-kb Kpnl fragment [Chattopadhyay and Ghosh, 1988a] have been cloned in pUC 19 at the Kpnl site. Two recombinant plasmids, pSR216 and pSR112, produced four of the five tRNA species (arginine, isoleucine, tyrosine, and tryptophan) encoded in the phage genome. The tRNA genes were located on a 1.45-kb Kpn1-HindIII subfragment.

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Deposited On:08 Sep 2011 12:12
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