Transgenic indica rice expressing mirabilis jalapa antimicrobial protein (Mj-AMP2) shows enhanced resistance to the rice blast fungus magnaporthe oryzae

Deo Prasad, Bishun ; Jha, Sanjay ; Bhushan Chattoo, Bharat (2008) Transgenic indica rice expressing mirabilis jalapa antimicrobial protein (Mj-AMP2) shows enhanced resistance to the rice blast fungus magnaporthe oryzae Plant Science, 175 (3). pp. 364-371. ISSN 0168-9452

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.plantsci.2008.05.015

Abstract

Mj-AMP2, a knottin-type antimicrobial peptide, in vitro inhibits the growth of several plant pathogenic fungi including Magnaporthe oryzae. We demonstrate that transgenic rice (Oryza sativa L.) plants expressing the Mj-AMP2 gene show enhanced resistance to M. grisea, the causal agent of the rice blast disease. Mj-AMP2 was efficiently expressed and the level of Mj-AMP2 ranged from 0.32% to 0.38% of the total protein in the transgenic rice plants. In vitro inhibitory activity assays with the crude protein extract from transgenic rice indicated that the Mj-AMP2 protein produced was biologically active. Constitutive expression of Mj-AMP2 in transgenic rice reduces the growth of M. grisea by 63% with respect to untransformed control plant, and no effect on plant phenotype was observed. Transgene expression of Mj-AMP2 gene was not accompanied by an induction of pathogenesis-related (PR) gene expression indicating that the transgene product itself is directly active against the pathogen. The results presented in this study suggest that the Mj-AMP2 gene could be a useful candidate for protection of rice plants against the rice blast fungus M. grisea.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Mj-AMP2; Genetic Transformation; Pusa Basmati 1; Magnaporthe oryzae; Knottin; Ubl
ID Code:60112
Deposited On:08 Sep 2011 10:01
Last Modified:08 Sep 2011 10:01

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