Identification of RAPD markers linked to a major blast resistance gene in rice

Naqvi, Naweed I. ; Michael Bonman, J. ; Mackill, David J. ; Nelson, Rebecca J. ; Chattoo, Bharat B. (1995) Identification of RAPD markers linked to a major blast resistance gene in rice Molecular Breeding, 1 (4). pp. 341-348. ISSN 1380-3743

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Rice blast, caused byPyricularia grisea, is a major production constraint in many parts of the world. The Korean rice variety Tongil showed high levels of resistance for about six years when widely planted under highly disease-conducive conditions, before becoming susceptible. Tongil was found to carry a single dominant gene, designatedPi-10t, conferring resistance to isolate 106 of the blast pathogen from the Philippines. We report here the use of bulked segregant RAPD analysis for rapid identification of DNA markers linked toPi-10t. Pooled DNA extracts from five homozygous blast-resistant (RR) and five susceptible (rr) BC3F2 plants, derived from a CO39 × Tongil cross, were analyzed by RFLP using 83 polymorphic probes and by RAPD using 468 random oligomers. We identified two RAPD markers linked to thePi-10t locus: RRF6 (3.8 ± 1.2 cM) and RRH18 (2.9 ± 0.9 cM). Linkage of these markers withPi-10t was verified using an F2 population segregating forPi-10t. The two linked RAPD markers mapped 7 cM apart on chromosome 5. Chromosomal regions surrounding thePi-10t gene were examined with additional RFLP markers to define the segment introgressed from the donor genome.Pi-10t is likely to be a new blast-resistance locus, because no other known resistance gene has been mapped on chromosome 5. These tightly linked RAPD markers could facilitate early selection of thePi-10t locus in rice breeding programmes.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Gene Mapping; Near-isogenic Lines; Oryza Sativa L.; Pyricularia Grisea Cav.; Pyricularia Oryzae Cav
ID Code:60094
Deposited On:08 Sep 2011 09:57
Last Modified:08 Sep 2011 09:57

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