Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice

Naqvi, Naweed I. ; Chattoo, Bharat B. (1996) Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice Genome, 39 (1). pp. 26-30. ISSN 0831-2796

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Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/g9...

Related URL: http://dx.doi.org/10.1139/g96-004

Abstract

Two randomly amplified polymorphic DNA (RAPD) markers, OPF62700 and OPH182400, tightly linked to Pi-10, a dominant blast-resistance gene conferring complete resistance to isolate 106 (international race IB46) of the blast fungus were identified. To derive sequence characterized amplified regions (SCARs) from OPF62700 and OPH182400, these amplified RAPD products were cloned and sequenced. Nucleotide sequence information, obtained for each end of the two linked RAPD markers, was used to design 24-mer oligonucleotide primers for PCR amplification of the respective SCARs. Polymorphisms appearing as differences in the length of the SCAR's alternate alleles were considered for the indirect selection of Pi-10. Such polymorphisms converted the linked dominant RAPD loci into codominant SCAR markers and also facilitated the indirect scoring of the blast-resistant and blast-susceptible genotypes. The development of length variant codominant SCAR markers linked to a major gene for blast resistance in rice is described. The codominant SCARs will facilitate marker-assisted selection of the Pi-10 locus in rice breeding programs and will also be useful as genetic markers for high resolution mapping of the Pi-10 region.

Item Type:Article
Source:Copyright of this article belongs to NRC Research Press.
Keywords:SCAR; Pyricularia Grisea; Oryza Sativa L.,; Blast Resistance; Indirect Selection
ID Code:60086
Deposited On:08 Sep 2011 09:57
Last Modified:08 Sep 2011 09:57

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