Recombinant glycoprotein based vaccine for Chandipura virus infection

Venkateswarlu, C. H. ; Arankalle, V. A. (2009) Recombinant glycoprotein based vaccine for Chandipura virus infection Vaccine, 27 (21). pp. 2845-2850. ISSN 0264-410X

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Chandipura virus (CHPV) has emerged as an important pediatric encephalitis-causing pathogen with very high mortality in India. No specific vaccine or treatment is available till date. We attempted to prepare a candidate vaccine employing recombinant CHPV Glycoprotein (rGp). The Glycoprotein gene (G-gene) of CHPV was expressed using Baculovirus expression system. The rGp was purified by HPLC and used for mice immunization, 3 doses, and 4 weeks apart. One microgram rGp was found to be optimum. Sero-conversion was observed as early as 2nd week by detecting anti-CHPV IgG antibodies. Antibody titres were immunogen-concentration dependent. Intracerebral challenge of the immunized mice with 100 LD50 of the homologous strain demonstrated 90% protection. In in vitro neutralization, antibodies from the immunized mice were able to neutralize heterologous viruses. There was 60% T cell proliferation observed against rGp in immunized mice. The study shows that rGp induces both arms of immune response and represents an ideal vaccine candidate for further evaluations.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Chandipura Virus; G Gene; Recombinant Vaccine; Baculovirus Expression System
ID Code:59794
Deposited On:07 Sep 2011 14:41
Last Modified:07 Sep 2011 14:41

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