Hepatitis E antibody profiles in serum and urine

Joshi, M. S. ; Walimbe, A. M. ; Arankalle, V. A. ; Chadha, M. S. ; Chitambar, S. D. (2002) Hepatitis E antibody profiles in serum and urine Journal of Clinical Laboratory Analysis, 16 (3). pp. 137-142. ISSN 0887-8013

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jcla.10...

Related URL: http://dx.doi.org/10.1002/jcla.10027


The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 26 healthy individuals were tested by recombinant HEV protein (55 kD)-based indirect enzyme-linked immunosorbent assay (ELISA). Uronegativity for anti-HEV IgM was noted in 71 (100%) serologically confirmed patients with hepatitis E. Hepatitis E patients (10/10) showed urinary absence or very low levels of total IgM by capture ELISA, suggesting absence or low levels of filtration, and/or local synthesis, and/or transudation of IgM in urine during infection. When these patients were tested for total IgG and IgA, microquantities of immunoglobulins were noted in all urine samples (10/10 for each). However, the proportions of uropositivity for anti-HEV IgG and IgA in hepatitis E patients were low and indicated only 21.42% and 49.33% concordance with seropositivity, respectively. Control groups also showed low and variable uropositivity for anti-HEV IgG and IgA. Overall, HEV-specific antibodies exhibited by serum in recent and past infections were not found in urine. The study demonstrated the inadequacy of urine specimens for detection of hepatitis E antibodies.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Hepatitis E; Anti-HEV Antibodies; Urinary Antibodies; Immunoglobulins; Pprotein
ID Code:59786
Deposited On:07 Sep 2011 14:36
Last Modified:07 Sep 2011 14:36

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