Hepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region

Arankalle, Vidya A. ; Deshmukh, Tejaswini M. ; Chobe, Leenata P. ; Chadha, Mandeep S. ; Walimbe, Atul M. (2001) Hepatitis G virus infection in India: prevalence and phylogenetic analysis based on 5' non-coding region Indian Journal of Gastroenterology, 20 (1). pp. 13-17. ISSN 0254-8860

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Objectives: To determine the prevalence of hepatitis G virus (HGV) infection in western India and to carry out phylogenetic analysis of HGV isolates. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) assay was used to detect HGV RNA in serum samples obtained from paid plasma donors, patients with hemophilia and voluntary blood donors. Nine Indian and one Kenyan HGV RNA-positive samples were sequenced in the 5' non-coding region (5'-NCR). Phylogenetic analysis based on the comparison of a 101 nucleotide fragment from a large number of HGV isolates from 22 countries (including Indian and Kenyan sequences obtained during the present study) was carried out. Results: HGV RNA positivity rates among paid plasma donors from a commercial plasmapheresis unit (7/43, 16.3%) and patients with hemophilia (5/44, 11.4%) were significantly higher than that in voluntary blood donors (0/51; p=0.003 and 0.019, respectively). Among patients with acute non-A to E hepatitis and fulminant hepatic failure, 1 of 50 and 1 of 28 were HGV RNA-positive, whereas 6 of 49 (12%) patients with chronic liver disease had circulating HGV RNA. All Indian isolates belonged to genotype 2, whereas the Kenyan isolate formed a distinct branch within genotype 1 consisting of African isolates. Conclusion: Our results suggest existence of parenteral transmission of HGV in the Indian population. HGV was not an important cause of acute non-A to E hepatitis or fulminant hepatic failure among the patients investigated. Genotype 2 seems to be the most prevalent genotype in western India.

Item Type:Article
Source:Copyright of this article belongs to Indian Society of Gastroenterology.
Keywords:GBV-C/HGV; Genotypes; Viral Hepatitis
ID Code:59784
Deposited On:07 Sep 2011 14:36
Last Modified:18 May 2016 10:14

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