Indigenous anti-hepatitis A virus IgM capture ELISA for the diagnosis of hepatitis A

Chitambar, S. D. ; Murthy-Grewal, S. ; Bokil, M. ; Arankalle, V. A. ; Gore, M. M. ; Banerjee, K. (1994) Indigenous anti-hepatitis A virus IgM capture ELISA for the diagnosis of hepatitis A Indian Journal of Medical Research, 99 . pp. 243-251. ISSN 0019-5340

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Anti-hepatitis A virus IgM capture ELISA was developed by using the reagents produced in the NIV laboratory. The major reagents of the assay were anti-human IgM antibody, hepatitis A virus (HAV) and anti-HAV IgG-horse radish peroxidase (HRP) conjugate. Of these, anti-human IgM antibodies were generated in rabbit against IgM secreted by human hybridoma clone(G3). HAV was derived from buffalo green money kidney cell line infected with HM-175 strain. Virus purified from the cell lysates was used for immunization of rabbits and guinea-pigs. There was very low anti-HAV response. A seropositive rhesus monkey was inoculated with monkey adapted strain of HAV to boost the anti-HAV antibody titre. Anti-HAV IgGs derived from hyperimmune sera of monkey and hepatitis A patient were conjugated with HRP. The preparations of conjugate--particularly human antibody--HRP conjugate yielded highly satisfactory results in anti-HAV capture ELISA. The assay appears to be specific, sensitive and quick and is useful in differentiating acute HAV infection from other acute infections caused by B, E and non-A non-B hepatitis viruses.

Item Type:Article
Source:Copyright of this article belongs to Indian Council of Medical Research.
ID Code:59782
Deposited On:07 Sep 2011 14:33
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