Evaluation of urine as a clinical specimen for diagnosis of hepatitis A

Joshi, Madhuri S. ; Chitambar, Shobha D. ; Arankalle, Vidya A. ; Chadha, Mandeep S. (2002) Evaluation of urine as a clinical specimen for diagnosis of hepatitis A Clinical and Diagnostic Laboratory Immunology, 9 (4). pp. 840-845. ISSN 1071-412X

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Official URL: http://cvi.asm.org/cgi/content/abstract/9/4/840

Related URL: http://dx.doi.org/10.1128/CDLI.9.4.840-845.2002


The present study pertains to the evaluation of urine as a specimen for detection of anti-hepatitis A virus (anti-HAV) antibodies. Immunoglobulin M (IgM), IgG, and IgA capture enzyme-linked immunosorbent assays for hepatitis A were performed on paired serum and urine specimens collected from hepatitis A patients (n=92), healthy individuals (n=100), non-A hepatitis patients (n=70), and patients with nonhepatic diseases (n=64, including 37 renal disease patients). Hepatitis A patients seropositive for anti-HAV IgM showed 95.65% uropositivity. No false-positive reactions were observed in control groups. The uropositivity of anti-HAV IgM persisted during the convalescent phase of the disease. Anti-HAV IgG uropositivity correlated well with corresponding seropositivity in all groups (P > 0.05 for each). No significant difference between the proportions of serum and urine positivity for anti-HAV IgA was noted (P > 0.05 for each). Using seroreactivity as a "gold standard", the sensitivity and specificity for anti-HAV IgM, anti-HAV IgG, and anti-HAV IgA tests with urine as a specimen were found to be 95.65 and 100%, 97.76 and 76.47%, and 92.23 and 88.18%, respectively. Urine appears to be comparable to serum for diagnosis of recent and past infection with hepatitis A.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:59780
Deposited On:07 Sep 2011 14:37
Last Modified:07 Sep 2011 14:37

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