The 'K' selected oligophilic bacteria: a key to uncultured diversity?

Watve, M. ; Shejval, V. ; Sonawane, C. ; Rahalkar, M. ; Matapurkar, A. ; Shouche, Y. ; Patole, M. ; Phadnis, N. ; Champhenkar, A. ; Damle, K. ; Karandikar, S. ; Kshirsagar, V. ; Jog, M. (2000) The 'K' selected oligophilic bacteria: a key to uncultured diversity? Current Science, 78 (12). pp. 1535-1542. ISSN 0011-3891

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Molecular techniques have made it increasingly clear that a large proportion of bacterial diversity in natural habitats is uncultured and therefore unexplored. We suggest and give evidence in support of a hypothesis that a large proportion, if not all, of the uncultured diversity from a variety of aquatic and terrestrial habitats are oligophilic (oligotrophic) bacteria. Oligophilic bacteria grow only on dilute nutrient media and form small or microscopic colonies. A technique to cultivate and isolate the moderately oligophilic bacteria was developed and 90 cultures isolated, The twelve bacterial cultures characterized showed high growth yield coefficients and carbon conversion efficiencies at low substrate concentrations and progressively decreased with increasing substrate concentrations. Most of the growth yields were substantially higher than those reported in the literature and lie near the theoretical maximum. Slow growth rates and high yields indicate that they are 'K' selected species. 16S rDNA partial sequence analysis of the isolates indicates that it is a novel as well as diverse group.

Item Type:Article
Source:Copyright of this article belongs to Current Science Association.
ID Code:59714
Deposited On:20 Jun 2012 13:48
Last Modified:18 May 2016 10:11

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