An integrated physical and genetic map of the rice genome

Chen, Mingsheng ; Presting, Gernot ; Barbazuk, W. Brad ; Goicoechea, Jose Luis ; Blackmon, Barbara ; Fang, Guangchen ; Kim, Hyeran ; Frisch, David ; Yu, Yeisoo ; Sun, Shouhong ; Higingbottom, Stephanie ; Phimphilai, John ; Phimphilai, Dao ; Thurmond, Scheen ; Gaudette, Brian ; Li, Ping ; Liu, Jingdong ; Hatfield, Jamie ; Main, Dorrie ; Farrar, Kasey ; Henderson, Caroline ; Barnett, Laura ; Costa, Ravi ; Williams, Brian ; Walser, Suzanne ; Atkins, Michael ; Hall, Caroline ; Budiman, Muhammad A. ; Tomkins, Jeffery P. ; Luo, Meizhong ; Bancroft, Ian ; Salse, Jerome ; Regad, Farid ; Mohapatra, Trilochan ; Singh, Nagendra K. ; Tyagi, Akhilesh K. ; Soderlund, Carol ; Dean, Ralph A. ; Wing, Rod A. (2002) An integrated physical and genetic map of the rice genome The Plant Cell Online, 14 (3). pp. 537-545. ISSN 1040-4651

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Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome fingerprint-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome (~90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes.

Item Type:Article
Source:Copyright of this article belongs to American Society of Plant Biologists.
ID Code:57952
Deposited On:30 Aug 2011 10:17
Last Modified:30 Aug 2011 10:17

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