Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component

Karthikeyan, A. S. ; Vanitharani, R. ; Balaji, V. ; Anuradha, S. ; Thillaichidambaram, P. ; Shivaprasad, P. V. ; Parameswari, C. ; Balamani, V. ; Saminathan, M. ; Veluthambi, K. (2004) Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component Archives of Virology, 149 (8). pp. 1643-1652. ISSN 0304-8608

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Official URL: http://www.springerlink.com/content/lamjejhvatlw2f...

Related URL: http://dx.doi.org/10.1007/s00705-004-0313-z

Abstract

One DNA A (KA30) and five different DNA B components (KA21, KA22, KA27, KA28 and KA34) of a geminivirus, Mungbean yellow mosaic virus-Vigna (MYMV-Vig) were cloned from a pooled sample of field-infected Vigna mungo plants from Vamban, South India. MYMV-Vig DNA A (KA30) and one of the DNA B components (KA27) exhibited 97% and 95% sequence identities, respectively, to those of MYMV reported from Thailand. However, the DNA B components KA21, KA22, KA28 and KA34 exhibited only 71 to 72% sequence identity to MYMV DNA B. Co-existence of multiple DNA B components in field-infected V. mungo was proved by Southern and PCR analyses. Each of the five DNA B components was infective together with the DNA A upon agroinoculation. Agroinoculation with mixed cultures of Agrobacterium with partial dimers of DNA A and all five DNA Bs proved that all five DNA B components can co-infect a single V. mungo plant.

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