Rice scutellum induces Agrobacterium tumefaciens vir genes and T-strand generation

Vijayachandra, K. ; Palanichelvam, K. ; Veluthambi, K. (1995) Rice scutellum induces Agrobacterium tumefaciens vir genes and T-strand generation Plant Molecular Biology, 29 (1). pp. 125-133. ISSN 0167-4412

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Official URL: http://www.springerlink.com/content/v062521622j712...

Related URL: http://dx.doi.org/10.1007/BF00019124


For successful transformation of a plant by Agrobacterium tumefaciens it is essential that the explant used in cocultivation has the ability to induce Agrobacterium tumour-inducing (Ti) plasmid virulence (vir) genes. Here we report a significant variation in different tissues of Indica rice (Oryza sativa L. cv. Co43) in their ability to induce Agrobacterium tumefaciens vir genes and T-strand generation, using explants preincubated in liquid Murashige and Skoog (MS) medium. An analysis of rice leaf segments revealed that they neither induced vir genes nor inhibited vir gene induction. Of different parts of rice plants of different ages analysed only scutellum from four-day old rice seedlings induced vir genes and generation of T-strands. We observed that the physical presence of preincubated scutella is required for vir gene induction. Conditioned medium from which preincubated scutella were removed did not induce the vir genes. Scutellum-derived calli, cultured for 25 days on medium containing 2,4-D, also induced virE to an appreciable level. These results suggest that scutellum and scutellum-derived calli may be the most susceptible tissues of rice for Agrobacterium-mediated transformation.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Agrobacterium tumefaciens; Plant Transformation; Rice; Scutellum; T-strands; vir Genes
ID Code:57226
Deposited On:26 Aug 2011 03:40
Last Modified:26 Aug 2011 03:40

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