Agrobacterium tumefaciens-mediated transformation of Vigna mungo (L.) Hepper

Karthikeyan, A. S. ; Sarma, K. S. ; Veluthambi, K. (1996) Agrobacterium tumefaciens-mediated transformation of Vigna mungo (L.) Hepper Plant Cell Reports, 15 (5). pp. 328-331. ISSN 0721-7714

Full text not available from this repository.

Official URL: http://www.springerlink.com/content/vg1h120l7w05j3...

Related URL: http://dx.doi.org/10.1007/BF00232365

Abstract

Transformed Vigna mungo (blackgram) calli were obtained by cocultivating segments of primary leaves with Agrobacterium tumefaciens vir helper strains harbouring the binary vector pGA472 having kanamycin resistance gene as plant transformation marker. Transformed calli were selected on Murashige and Skoog medium supplemented with 50 mg/l kanamycin and 500 mg/l carbenicillin. Transformed calli were found to be resistant to kanamycin up to 900 mg/l concentration. Expression of kanamycin resistance gene in transformed calli was demonstrated by neomycin phosphotransferase assay. Stable integration of transferred DNA into V. mungo genome was confirmed by Southern blot analysis.

Item Type:Article
Source:Copyright of this article belongs to Springer.
ID Code:57225
Deposited On:26 Aug 2011 03:40
Last Modified:26 Aug 2011 03:40

Repository Staff Only: item control page