Tyagi, Jaya Sivaswami ; Pastan, Ira (1990) Purification o calf thymus RNA polymerase II for in vitro transcription studies Journal of Biochemical and Biophysical Methods, 21 (1). pp. 1-8. ISSN 0165-022X
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-022X(90)90039-F
Abstract
A procedure for the purification of a large amount of RNA polymease II (RNAP II) from calf thymus is described. This procedure results in an approximately 1400-fold purification with 40% yield of enzyme in 60 h. The partially purified enzyme is highly suitable for in vitro transcription studies in a cell-free system utilizing HeLa S-100. This method for RNAP II purification would find significant applications in in vitro studies for the analysis of factors modulating eukaryotic transcription.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | RNA polymease; Transcription |
| ID Code: | 57213 |
| Deposited On: | 26 Aug 2011 02:22 |
| Last Modified: | 26 Aug 2011 02:22 |
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