Ramanathan, Vai. ; Veluthambi, K. (1995) Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA Plant Molecular Biology, 28 (6). pp. 1149-1154. ISSN 0167-4412
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Official URL: http://www.springerlink.com/content/h649268r856231...
Related URL: http://dx.doi.org/10.1007/BF00032676
Abstract
We introduced a plant selection marker, nptII, to the left of border A in the Agrobacterium Ti plasmid pTiA6. Infection of tobacco leaf discs with the modified Agrobacterium strain gave rise to kanamycin-resistant calli which grew in a hormone-dependent manner. Southern hybridization analysis of DNA isolated from four transformants indicated initiation of DNA transfer at or near border A and absence of T-DNA sequences. These results demonstrate that DNA transfer events starting at a left border on a native Ti plasmid and moving away from the T-DNA region occur and that they can be detected by designing a suitable selection strategy.
Item Type: | Article |
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Source: | Copyright of this article belongs to Springer. |
Keywords: | Agrobacterium tumefaciens; T-DNA Transfer |
ID Code: | 57049 |
Deposited On: | 25 Aug 2011 12:44 |
Last Modified: | 25 Aug 2011 12:44 |
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