Studies on the tryptophan residues of soybean agglutinin. Involvement in saccharide binding

Swamy, Musti Joginadha ; Surolia, Avadhesha (1989) Studies on the tryptophan residues of soybean agglutinin. Involvement in saccharide binding Bioscience Reports, 9 (2). pp. 189-198. ISSN 0144-8463

Full text not available from this repository.

Official URL: http://www.springerlink.com/content/m5240863v40m51...

Related URL: http://dx.doi.org/10.1007/BF01115995

Abstract

Modification of tryptophan side chains of soybean agglutinin (SBA) with N-bromosuccinimide results in a loss of the hemagglutinating and carbohydrate binding activities of the protein. One residue/subunit is probably essential for the binding activity. Modification leads to a large decrease in the fluorescene of the protein accompained by a blue shift. Iodide ion quenching of the protein fluorescence shows that saccharide binding results in a decreased accessibility of some of the tryptophan side chains. These results strongly point towards the involvement of tryptophan residues in the active site of SBA.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Saccharide Binding; Soybean Agglutinin; Tryptophan
ID Code:56484
Deposited On:24 Aug 2011 11:26
Last Modified:04 Jul 2012 08:40

Repository Staff Only: item control page