Subcellular localization of a protein-tyrosine phosphatase: evidence for association with chromatin

Radha, Vegesna ; Nambirajan, Sundaram ; Swarup, Ghanshyam (1994) Subcellular localization of a protein-tyrosine phosphatase: evidence for association with chromatin Journal of Biochemistry, 299 . pp. 41-47. ISSN 0021-924X

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Official URL: http://www.biochemjusa.org/bj/299/0041/2990041.pdf

Abstract

A non-transmembrane protein-tyrosine phosphatase, PTP-S, has bee shown to bind to DNA in vitro through its C-terminal noncatalytic domain. The cellular PTP-S gene product was identified a. 42-44 kDa polypeptides in the rat fibroblast cell lines Rat2 and Fl 1 1, by immunoblotting using polyclonal and monoclonal antibodies. Indirect immunofluorescence-staining experiments showed that PTP-S was predominantly associated with the nuclear compartment in interphase cells, although some fluorescence was present in cytosplasm. This protein appeared to be uniformly distributed throughout the cell during mitosis. Subcellular fractionation followed by immunoblotting supports the immunofluorescence data showing that PTP-S was present in the nucleus as well as cytoplasm. PTP-S could be released from the isolated nuclei by moderate salt concentration (400 mM NaCl) or mild treatment with DNAase I, suggesting that at least part of this protein present in the nucleus was associated with chromatin. PTP-S extracted from nuclei of Rat2 cells binds to DNA, as determined by DNA affinity chromatography. The nuclear location of this phosphatase, and its association with chromatin, provide support to our previous suggestion that the DNAbinding property of this phosphatase may be physiologically relevant.

Item Type:Article
Source:Copyright of this article belongs to Oxford University Press.
ID Code:55913
Deposited On:19 Aug 2011 07:55
Last Modified:19 Aug 2011 07:55

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