Rajasekharan, R. ; Kemp, J. D. (1994) Synthesis of photoreactive phosphatidylethanolamine and its interaction with phospholipase A2 The Journal of Lipid Research, 35 . pp. 45-50. ISSN 0022-2275
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Official URL: http://www.jlr.org/content/35/1/45.short
Abstract
A photoreactive derivative of phosphatidylethanolamine, N-(4-azidobenzoyl)phosphatidylethanolamine (AB-PE), was synthesized by acylation of phosphatidylethanolamine with an N-hydroxysuccinimide ester of 4-azidobenzoic acid. The substantial photosensitivity exhibited by AB-PE correlated with a marked decrease in the absorption spectra of the compound. The compound proved sensitive to lipase and phospholipase A2 hydrolysis but resistant to phospholipase C and D activities. Photolysis of a sonicated dispersion of AB-PE containing phospholipase A2 resulted in irreversible inhibition of the enzyme. Addition of natural phosphatidylethanolamine provided protection against photoinactivation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Lipid Research, Inc. |
| Keywords: | Azido-phospholipids; Desaturation; Photo-affinity Label |
| ID Code: | 55069 |
| Deposited On: | 17 Aug 2011 12:16 |
| Last Modified: | 17 Aug 2011 12:16 |
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