High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice

Ashraf, Shadma ; Singh, P. K. ; Yadav, Dinesh K. ; Shahnawaz, Md. ; Mishra, Satish ; Sawant, Samir V. ; Tuli, Rakesh (2005) High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice Journal of Biotechnology, 119 (1). pp. 1-14. ISSN 0168-1656

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.jbiotec.2005.06.009

Abstract

A synthetic gene coding for the surface glycoprotein (G protein) of rabies virus was strategically designed to achieve high-level expression in transgenic plants. The native signal peptide was replaced by that of the pathogenesis related protein, PR-S of Nicotiana tabacum. An endoplasmic reticulum retention signal was included at C-terminus of the G protein. Tobacco plants were genetically engineered by nuclear transformation. Selected transgenic lines expressed the chimeric G protein at 0.38% of the total soluble leaf protein. Mice immunized intraperitoneally with the G protein purified from tobacco leaf microsomal fraction elicited high level of immune response as compared to the inactivated commercial viral vaccine. The plant-derived G protein induced complete protective immunity in mice against intracerebral lethal challenge with live rabies virus. The results establish that plants can provide a safe and effective production system for the expression of immunoprotective rabies virus surface protein.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Anti-rabies Vaccine; Immunoprotection; Plant Vaccine; Rabies G Protein
ID Code:54592
Deposited On:12 Aug 2011 07:00
Last Modified:12 Aug 2011 07:00

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