Tiwari, Siddharth ; Tuli, Rakesh (2009) Multiple shoot regeneration in seed-derived immature leaflet explants of peanut (Arachis hypogaea L.) Scientia Horticulturae, 121 (2). pp. 223-227. ISSN 0304-4238
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.scienta.2009.01.029
Abstract
A protocol was developed for organogenesis from immature leaflet explants derived from mature seeds of peanut. Immature leaflets pre-incubated on MS medium supplemented with 13.32 μM BAP + 4.95 μM NAA for 7 days, turned green and enlarged. The enlarged green leaflets produced multiple shoot buds after 1-2 cycles of sub-culture on MS medium supplemented with 13.32 μ M BAP. Three cycles of shoot buds on the elongation medium (13.32 μM BAP) produced 6.17 ± 0.47 elongated shoots per explant. The shoot bud formation was genotype independent. All elongated shoots rooted on the medium containing 4.95 μM NAA. The complete protocol gave efficient (>81%) direct organogenesis, leading to the development of plantlets within 4 months.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Organogenesis; Peanut; Phytohormone; Plant Regeneration |
| ID Code: | 54586 |
| Deposited On: | 12 Aug 2011 07:05 |
| Last Modified: | 12 Aug 2011 07:05 |
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