Tyagi, Anil K. ; Vishwakarma, Ram A. (1998) Recombinant Bacillus subtilis whole cell system as a catalyst for enzymatic synthesis of cyclic inositol phosphate Tetrahedron Letters, 39 (33). pp. 6069-6072. ISSN 0040-4039
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/S0040-4039(98)01250-7
Abstract
Whole cell culture of recombinant bacteria Bacillus subtilis, over expressing the phosphoinositide specific phospholipase C (PI-PLC) gene of Bacillus thuringiensis has been used as an efficient catalyst for practical multigram scale synthesis of D-myo-inositol 1,2-cyclic monophosphate directly from phospholipid mixtures containing phosphatidyl-inositol. This synthesis does not require protein purification and growing bacterial culture can be directly used. The reaction can be monitored by in-situ31P NMR.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 54567 |
| Deposited On: | 12 Aug 2011 07:09 |
| Last Modified: | 12 Aug 2011 07:09 |
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