Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors

Tyagi, Anil K. ; Jayaram, Hiremagular N. ; Anandaraj, Spurgeon ; Taylor, Benjamin ; Cooney, David A. (1979) Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precurosors Journal of Biochemical and Biophysical Methods, 1 (4). pp. 221-226. ISSN 0165-022X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0165-022X(79)90008-3

Abstract

A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5-100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Nucleic Acid Bases; Nucleosides; Nucleotides; Chromatography; Lithium Citrate; Lithium Chloride
ID Code:54563
Deposited On:12 Aug 2011 07:08
Last Modified:12 Aug 2011 07:08

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