Purification and characterization of restriction endonuclease MgoI from Mycobacterium gordonae

Shankar, Sandeep ; Tyagi, Anil K. (1993) Purification and characterization of restriction endonuclease MgoI from Mycobacterium gordonae Gene, 131 (1). pp. 153-154. ISSN 0378-1119

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0378-1119(93)90686-W

Abstract

A restriction endonuclease, MgoI, an isoschizomer of Sau3AI, was purified from Mycobacterium gordonae TRC1318. As compared to Sau3AI, the yield of MgoI was seven-eightfold higher, the enzyme was four times more stable at 37°C, and in addition, had optimal activity over a much broader range of salt concentrations.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Recombinant DNA; Sau3 Ai Isoschizomer; Mycobacteria
ID Code:54550
Deposited On:12 Aug 2011 07:09
Last Modified:12 Aug 2011 07:09

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