Activity of the yeast FLP recombinase in Arabidopsis

Sonti, Ramesh V. ; Tissier, Alain F. ; Wong, Donny ; Viret, Jean-Frédéric ; Signer, Ethan R. (1995) Activity of the yeast FLP recombinase in Arabidopsis Plant Molecular Biology, 28 (6). pp. 1127-1132. ISSN 0167-4412

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The coding sequence for FLP recombinase, originally from the 2μ plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Arabidopsis; FLP Recombinase; Site-specific Recombination
ID Code:54152
Deposited On:11 Aug 2011 11:35
Last Modified:11 Aug 2011 11:35

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